An innovative new screening method using cell aggregates shaped like spheres may lead to the discovery of smarter cancer drugs, a team from the Scripps Research Institute, California, US, has reported.
The 3D aggregates, called spheroids, can be used to obtain data from potentially thousands of compounds using high throughput screening (HTS). HTS can quickly identify active compounds and genes in a specific biomolecular pathway using robotics and data processing.
A spheroid under a confocal microscope. Image: Kota et al./The Scripps Research Institute
The spheroids – 100 to 600 microns thick in diameter – spread in a similar way to cancer cells in the body and are therefore more effective in identifying potential cancer drugs, the team hypothesises.
For this study, the team focused on KRAS – a gene belonging to the RAS family. It is estimated these genes account for one-third of all cancers.
Robots handle assays in a HTS system. Image: NIH/Flickr