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Biotech Fluidics

Telephone: +46 300 56 91 80 / +1 612 7035718
Email: info@biotechfluidics.com
Web: biotechfluidics.com/products/detectors/runge-mikron-miniature-detectors/

Fluorescence detection module

Biotech Fluidics has added to its range of Runge Mikron modular detectors. With a diameter of just 32mm and measuring only 128mm in length, the Mikron 71 is the smallest commercially available fluorimeter.

It is available with multiple interchangeable fixed wavelength filters, light sources and flow cells enabling both simulation and emission fluorescence measurements.

Flexible modularity enables it to be configured for almost any application, particularly instruments lacking fluorescence detection such as preparative liquid chromatography systems.

With a long-life, low power LED light source, it can be used directly in a chilled environment at 4°C – making it perfect for bioanalytical applications such as detection of proteins down to the nanomolar level.

The reference channel ensures a high degree of accuracy and sensitivity without external calibration.

It is easy to connect as it communicates and draws power from a USB-C port. Drivers are provided for several popular chromatography software packages, alternatively an open protocol can be used.

TESTA

Telephone: +49-30-864-24076
Email: info@testa-analytical.com
Web: testa-analytical.com/gpc-sec-chromatography.html

Realtime monitoring of flow rate

A new study from TESTA Analytical demonstrates the importance of continuous monitoring of flow rate in GPC/SEC experiments.

Researchers investigated the effect of a +/- 1% deviation on results for an unknown sample determined with both a standard calibration and a universal calibration. This small variation was selected as it might arise due to backpressure in a typical GPC/SEC system, often below the detection limit of the pump pressure transducer and is thus undetectable with on-board means. The investigation was conducted by running a sample right after calibration, performing calculations and subsequently manually changing the flow rate parameter used for calculation. This was then repeated for a second sample using a viscosity detection and universal calibration.

The study showed a 1% deviation in flow rate resulted in a change of over 20% in calculated molecular weight results. This underlines the necessity for a suitable method of constant monitoring of current flow rates and correction of running chromatogram.